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KMID : 1145520170030010038
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Journal of Radiopharmaceuticals and Molecular Probes
2017 Volume.3 No. 1 p.38 ~ p.43
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A study of 99mTc-sestamibi labeling condition using radio-chromatography
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Moon Sung-Hyun
Lee Yun-Sang
Lee Dong-Soo
Chung June-Key
Jeong Jae-Min
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Abstract
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Tc-99m labeled sestamibi (99mTc-MIBI) is one of most widely used radiopharmaceuticals for myocardial SPECT imaging. Radiolabeling of 99mTc-MIBI is recommended by heating in 100°C water bath for 15 min. However, the water bath might be a source of contamination. Thus, if radiolabeling of 99mTc-sestamibi can be performed at room temperature, then it would be more convenient to use in clinical application. In this study, we performed the radiolabeling of 99mTc-MIBI in different temperature conditions or using different instruments to find out the efficient labeling condition. We studied the 99mTc-MIBI labeling at room temperature or 100oC heating block, and checked the labelling yields every 1 min for 10 min using radio-TLC with 2 different eluents?saline and acetone. From the experiment, we confirmed that the 99mTc-MIBI can be labeled over 90% yield but not completed at room temperature. However, the 99mTc-MIBI labeling was completed when it was performed in the 100oC heating block. Finally, we proved that heating is essential for complete 99mTc-MIBI labelling, furthermore using heating block is also possible instead of water bath.
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KEYWORD
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99mTc-MIBI, Sestamibi, Radio TLC, Labelling condition
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